MOD50
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 | Superior Sensitivity. Bisulfite modification was performed on decreasing concentrations of human genomic DNA using Imprint (Cat. No. MOD50) and kits from two other suppliers. Methylation-specific PCR (MSP) was then performed on all samples using β-actin gene-specific primers (109 bp amplicon). For all suppliers lanes from left to right are 100 ng, 10 ng, 1 ng, 100 pg, 50 pg and no sample, respectively. Suppliers C and D (not shown) have no product at 50 pg. These results demonstrate the superior sensitivity of the Imprint kit relative to other suppliers. |
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MOD50
Sigma
Imprint® DNA Modification Kit
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Features and Benefits | • Only 50 picograms of DNA or 20 cells are required • Procedure takes less than 2 hours • Greater than 99% conversion rate • Extremely low degradation • Option of convenient one-step protocol • Consistent and reproducible Bisulfite Modification |
| General description | The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray. |
| Legal Information | Imprint is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| usage | sufficient for 50 reactions |
| storage temp. | 20-25°C |
Safety
| Hazard Codes | C |
| Risk Statements | 22-31-35 |
| Safety Statements | 25-26-36/37/39-45-46 |
| RIDADR | UN 1824 9/PG 3 |
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References
| reference | Li, K.K., et al., EMP3 overexpression is associated with oligodendroglial tumors retaining chromosome arms 1p and 19q. Int. J. Cancer 120(4), 947-950, (2007) |
| Tong, Y.K., et al., Noninvasive prenatal detection of fetal trisomy 18 by epigenetic allelic ratio analysis in maternal plasma: Theoretical and empirical considerations. Clin. Chem. 52, 2182-2183, (2006) Abstract | |
| Cheng, S.H., et al., 4q loss is potentially an important genetic event in MM tumorigenesis: identification of a tumor suppressor gene regulated by promoter methylation at 4q13.3, platelet factor 4. Blood 109(5), 2089-2099, (2006) |
